DEVELOPMENT OF BIOPROCESSES FOR PRODUCTION AND PURIFICATION OF L-ASPARAGINASE FROM STAPHYLLOCOCCUS AUREUS, AND IN VITRO EFFECACY AGAINST HUMAN BREAST CNCER CELL LINE

The present study was aimed to isolate the abundant Staphylococcus aureus produce asparaginase and evaluate efficacy of purified L-ASNase against a breast cancer cell line. From one hundred local isolates S. which subjected primary screening processes for production, thirty-seven with maximum ability hydrolysis zone in (the ratio Z more than 10 mm) were selected secondary screening. It has been indicated that TG98 had highest productivity enzyme (255.8 U/mg protein). producing-isolate identified according vitek test. optimal conditions ASNase production by performed using submerged fermentation, where medium (5) utilized as best lactose carbon source, NH4Cl nitrogen pH 8 at 37 °C after 24 hr incubation period, specific activity reached 496.99 U/mg. gel filtration chromatography utilizing Sephadex G-150. results there is an elevating final purification folds 2 time 103% yield enzyme. Also, it exhibited maximal stability 8.0. Also active stable 37°C.The rate specificity found asparagine. tumor cells proliferation significantly suppressed cytotoxic effect comparison normal line (WRL-68).